Faecal microbiota transplantation‐mediated jejunal microbiota changes halt high‐fat diet‐induced obesity in mice via retarding intestinal fat absorptionIF:1.40

The jejunal mucosal scrapings were collected and immediately snap-frozen in liquid nitrogen and stored at  80°C.The QIAamp DNA stool Mini Kit (Qiagen, Hilden, Germany) was used to extract DNA from enteric bacteria injejunal mucosal scrapings and PCR amplification of theV3-V4 region (338F: ACTCCTACGGGAGGCAGCA,806R: GGACTACHVGGGTWTCTAAT) of 16S rDNA. Theamplification products were detected by 2% gel electrophoresis and PCR products were recovered by axyprepDNA gel extraction kit (Axygen, San Francisco, CA, USA).The PCR products were detected and quantified byQuantiFluorTM-ST microfluorometer (Promega, Madison,WI, USA) and then Miseq library was constructed forpyrosequencing on the Illumina MiSeq platform (Illumina,Madison, WI, USA). Clean sequences were assigned to
the same operational taxonomic units (OTUs) based on asimilarity ≥ 97%. Data were analysed on the free onlinecloud platform (https://cloud.majorbio.com/) of Majorbio(Shanghai, China). Bacterial species were further identi-fied by sequence comparison of OTUs on NCBI. The relative abundance of significantdifferences in phylum, genusand OTU levels was examined by the Kruskal–Wallis ranksum test. Spearman’s correlation analysis was furtheranalysed to uncover the relationship between jejunalmicrobiota and intestinal fat absorption.

感谢动物营养与饲料科学重点实验室（华东），农业农村部，浙江杭州 310058，中国。浙江省动物饲料与营养重点实验室，浙江杭州 310058，中国。浙江大学预防兽医研究所，浙江杭州 310058，中国。6浙江省预防兽医重点实验室，引用文献！