肠道菌增菌肉汤

成分(g/L)
Pancreatic Digest of Gelatin/
胰酶 明胶消化物             10.0
Glucose monohydrate /
葡萄糖                       5.0
Disodium hydrogen phosphate dihydrate/
磷酸氢二钾                   8.0
Potassium dihydrogen phosphate/
磷酸二氢钠                   2.0
Dehydrated Ox Bile/
脱水牛胆粉                  20.0
Brilliant Green/煌绿           0.015
pH值7.2 ± 0.2             25℃
用法
Suspend 45.0g / liter,and heat to boiling for 1  to  2 minutes. Transfer 120-ml  portions to  250-ml  volumetric flasks or 9-ml portions to test tubes, all being capped with cotton plugs or loose-fitting caps. Heat at 100 °C for 30 min and cool immediately.
称取本品 45.0g,加热搅拌溶解于 1000ml 蒸馏水中,分装三角瓶每瓶 120ml或分装试管 ,每管 9ml,100℃水浴加热 30 分钟 , 迅速在流动的冷水中冷却,无需高压灭菌。